Thamrin Jakart » Universitas Nusantara Manado » Universitas Parna Raya Manado » Universitas Patria Artha » Universitas Pelita Bangsa Beka » Universitas Surapati Jakarta » Universitas Tanri Abeng Jakart » Universitas Duta Bangsa » Universitas Halim Sanusi Bandu » Universitas Indonesia Mandiri » Universitas IVET Semarang » Universitas Kahuripan Kediri » Universitas Kristen Surakarta » Universitas Ma Chung Malang Bali Dwipa Denpasar » Universitas Al-Azhar Medan » Universitas Boyolali » Universitas Cokroaminoto Makas » Universitas Deli Sumatera » UM Surabaya » UMJ Jakarta » UMPTB Lampung » UNAKI Semarang » UNDARIS Ungaran Semarang » UNIBA Banyuwangi » UNIMUS Semarang » STT Sapta Taruna Jakarta » STT Yuppentek Tangerang » STTG Walisongo Gempol » STTG-STIEG Walisongo Gempol » UHAMZAH Medan » UICM Bandung » UM Palangkaraya Khez Muttaqien » STT Duta Bangsa Bekasi » STT Mandala Bandung » STT Muhammadiyah Cileungsi » STMIK MJ Matraman Jakarta » STT Bandung » STT Bina Tunggal Bekasi » STT Dr.
» STIKI Malang » STIMA IMMI Jakarta » STiPsi Yogyakarta » STIT Al-Hikmah Lampung » STIT Hidayatullah Batam » STMIK MJ Bekasi » STMIK MJ Ciracas Jakarta » STIE Pioneer Manado » STIE Trianandra Pemuda Jakarta » STIE Widya Darma Surabaya » STIE Widya Persada Jakarta » STIEG Walisongo Gempol » STIH Dharma Andigha Bogor » STIKES Sapta Bakti Bengkulu » STIE GICI Business School Beka » STIE GICI Business School Jaka » STIE Hidayatullah Depok » STIE IGI Jakarta » STIE Indocakti Malang » STIE Mitra Indonesia » STIE PEMUDA Surabaya » STIE ABI Surabaya » STIE Al-Rifaie Malang » STIE AMKOP Makassar » STIE Cendekia Semarang » STIE Ganesha Jakarta » STIE GEMA Bandung » STIE GICI Business School Dep » STAI Muhammadiyah Tulungagung » STAI Terpadu Yogyakarta » STEBI Bama Probolinggo » STEBI Bina Essa Bandung » STEBI Global Mulia Cikarang » STEI Yogyakarta » STIA Bayuangga Probolinggo » S2 FISIP UMJ Jakarta » S2 Geo Nusantara Bogor » S2 ITB Ahmad Dahlan Jakarta » S2 Mpu Tantular Jakarta » S2 UNKRIS Jakarta » STAI Duta Bangsa Bekasi » STAI Muhammadiyah Probolinggo
» MMT UNKRIS Jakarta » Mpu Tantular Jakarta » Mpu Tantular Kedoya Jakarta » MT Elektro JGU Jakarta » MT UNKRIS Jakarta » Polhas Surakarta » Polnas Denpasar » MM STIE GICI Business School » MM STIE IGI Jakarta » MM STIE Mitra Yogyakarta » MM STIMA IMMI Jakarta » MM Universitas Surapati Jakart » MM UNKRIS Jakarta » MM UNTARA Tangerang » MKom Geo Nusantara Bogor » MM Geo Nusantara Bogor » MM Mpu Tantular Jakarta » MM Patria Artha Makassar » MM Pelita Bangsa Bekasi » MM STIE ABI Surabaya » MM STIE Ganesha Jakarta » ITEKES Tri Tunas Nasional Maka » JGU Cibitung » JGU Jakarta » MH Mpu Tantular Cipinang Jakar » MH UNKRIS Jakarta » MIA FISIP UMJ Jakarta » MIKOM FISIP UMJ Jakarta » Institut Geo Nusantara » ISIF Cirebon » ISTA Jakarta » ITB Ahmad Dahlan Jakarta » ITB STIKOM Bali » ITB STIKOM Jimbaran Bali » ITBU Jakarta Thus, we demonstrate that MED26 strongly interacts with TFIID and recommend the use of a P11-DE52-M2 resin affinity three-step purification procedure to obtain MED26-free TFIID for analyzing Mediator-dependent transcription regulatory mechanisms in purified transcription systems in vitro.» Fakultas Pertanian UMJ Jakarta » FE MH Thamrin Jakarta » FISIP UMJ Jakarta » FK MH Thamrin Jakarta » IAI Abdullah Said Batam » IKIP Widya Darma Surabaya » IMWI Sukabumi We further show that a MED26-free TFIID complex can be obtained by including a simple additional DE52 chromatography step following P11 fractionation. Here we show first that the MED26 (CRSP70) coactivator subunit of Mediator co-purifies with TFIID in the above two-step protocol and interacts strongly with TFIID under high salt conditions. Purification of TFIID from HeLa nuclear extracts generally required a two-step purification procedure involving phosphocellulose P11 chromatography followed by anti-flag M2 affinity purification (Chiang et al., 1993 Ge et al., 1996). A breakthrough in TFIID purification was the generation of HeLa cell lines that express a FLAG epitope-tagged TBP subunit and immunopurification protocols with monoclonal anti-FLAG antibodies. Purification of TFIID free of other general transcription factors and coactivators is essential to analyze the transcription regulatory mechanisms in reconstituted systems in vitro. It is composed of the TATA box-binding protein (TBP) and ~13 different TBP-associated factors (TAFs). The general transcription factor TFIID is a multiprotein complex that is essential for specific transcription initiation by RNA polymerase II.